Study on Gallbladder Carcinoma Apoptosis Induced by Antisense Oligodeoxynucleotide Targeting survivin
- VernacularTitle:survivin反义寡核苷酸诱导胆囊癌细胞凋亡的研究
- Author:
Limin FENG
;
Xihong JIANG
;
Xinlin WU
;
Jianli WANG
;
Nanhai SHOU
- Publication Type:Journal Article
- Keywords:
Antisense oligodeoxynucleotide survivin Apoptosis Gallbladder carcinoma
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the apoptosis of gallbladder carcinoma cell line GBC-SD induced by antisense oligodeoxynucleotide (ASODN) targeting survivin. Methods ASODN targeting survivin was transfected into GBC-SD cells mediated by lipofectin. Cultured cells were divided into 3 groups: control group,sense oligonucleotide (SODN) group and ASODN group. After transfected for 16 h, the cultured cells were harvested and the following texts were carried out. The expression of survivin mRNA was detected by RT-PCR. Flow cytometer were used to detect apoptosis. Morphological changes were observed by electron microscopy. Results The expression of survivin mRNA was decreased 47.83% in ASODN group while apoptosis was increased from (0.50?0.23)% to (26.28? 3.91)%. Abnormal morphological changes of cells were observed in ASODN group and apoptosis bodies were found in some gallbladder carcinoma cells. Conclusion The expression of survivin may be decreased in GBC-SD cells after ASODN transfection.ASODN targeting survivin could induce gallbladder carcinoma cells apoptosis effectively.
