Characterization of memory T cells following stimulation with BCG
- VernacularTitle:人外周血卡介苗(BCG)特异性中央型和效应型记忆CD4~+ T细胞的特征
- Author:
Yanying FAN
;
Litao YANG
;
Li LI
;
Changyou WU
- Publication Type:Journal Article
- Keywords:
BCG;
Effector/central memory T cell;
IFN-?;
CCR7;
CD62L
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To elucidate the characterization of antigen-specific memory T cells from PPD~+ individuals after stimulation with BCG in vitro.Methods:PBMCs were isolated from PPD~ -/+ normal human peripheral blood and stimulated with BCG. The level of IFN-? in the culture supernatants was assayed by ELISA and the frequency of IFN-?-producinging cells was detected by ELISPOT. The subsets and frequency of cytokine-producing cells were determined at a single cell level by flow cytometry.Results:After stimulation with BCG, PBMCs from PPD~+ but not PPD~- individuals produced significantly high levels of IFN-? in culture supernatants detected by ELISA(P
