Effect of perforin-mediated cytotoxicity on systemic defense mechanisms against primary influenza virus infection
- VernacularTitle:穿孔素介导的细胞凋亡机制在抗流感病毒感染免疫防御中作用的研究
- Author:
Beixing LIU
;
Changlong LV
;
Yoshinobu KIMURA
- Publication Type:Journal Article
- Keywords:
Perforin;
Influenza virus;
Cytolytic activity;
Apoptosis
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of perforin-mediated cytotoxicity in primary influenza virus infection.Methods:Perforin-deficient and wild-type C57BL/6 mice were infected intranasally with influenza virus A/PR/8/34. Pulmonary viral growth was determined at various days after infection by pfu experiment. Perforin-mediated apoptotic degeneration was observed by Immunohistochemical staining. LDH-release method was used for detection of specific CTL and NK cell activity from spleen cells.Results:Mice deficient in the perforin gene showed an increased virus growth and prolonged virus shedding. The appearance of apoptotic degeneration in virally infected lung cells was delayed in perforin-deficient mice. The cytolytic activities of natural killer cells and virus-specific cytotoxic T lymphocytes were significantly lower than that of wild-type mice.Conclusion:Perforin plays a critical role in the host defense system against primary influenza virus infection.
