Regulation of bcl-2 family in hydrogen peroxide-induced apoptosis in human leukemia HL-60 cells.
- Author:
Jung Eun LEE
1
;
Jeong Won SOHN
;
Jung Hwa LEE
;
Kwang Chul LEE
;
Chang Sung SON
;
Young Chang TOCKGO
Author Information
1. Department of Pediatrics, Korea University, Seoul.
- Publication Type:Original Article
- Keywords:
hydrogen peroxide;
apoptosis;
HL-60 cell;
Bcl-2;
cell cycle
- MeSH:
Apoptosis/drug effects*;
Blotting, Western;
Cycloheximide/pharmacology;
DNA Fragmentation;
DNA, Neoplasm/metabolism;
DNA, Neoplasm/genetics;
DNA, Neoplasm/drug effects;
Dose-Response Relationship, Drug;
Flow Cytometry;
Gene Expression Regulation, Neoplastic/drug effects;
HL-60 Cells;
Human;
Hydrogen Peroxide/pharmacology*;
Oxidants/pharmacology*;
Protein Synthesis Inhibitors/pharmacology;
Proto-Oncogene Proteins/metabolism;
Proto-Oncogene Proteins/genetics;
Proto-Oncogene Proteins c-bcl-2/metabolism;
Proto-Oncogene Proteins c-bcl-2/genetics*;
RNA, Messenger/metabolism;
RNA, Messenger/genetics;
RNA, Messenger/drug effects
- From:Experimental & Molecular Medicine
2000;32(1):42-46
- CountryRepublic of Korea
- Language:English
-
Abstract:
Numerous types of cells have been shown to undergo apoptosis when exposed to oxidant agent such as hydrogen peroxide. In order to understand the functional relationship between the anti- and pro-apoptotic regulatory proteins in the cells under oxidant stress, we have studied the level of expression of apoptosis regulatory proteins, bcl-2 and bax, in human leukemia HL-60 cells. The exposure of HL-60 cells to different concentrations of H2O2 for 6 h resulted in a typical apoptosis of the cells as characterized by flow cytometry, cell cycle analysis, and DNA fragmantation. There was a block in G1 to S transition and apoptotic cells were mainly derived from S and G2 cells. Kinetic study demonstrated that the levels of both bcl-2-mRNA and -protein expression were decreased with the progression of cellular apoptosis whereas the level of bax-mRNA was unchanged but the expressed bax-protein was not detectable. Cycloheximide, a nonspecific translation inhibitor, did not prevent the hydrogen peroxide-mediated apoptosis in HL-60 cells. These results suggest that the regulation of bcl-2, but not of bax are important factor in the oxidative stress-induced apoptosis in HL-60 cells.
