Gene transfer with SonoVue and plasmid GFP into skeletal muscle cell
- VernacularTitle:超声造影剂SonoVue介导质粒绿色荧光蛋白转染小鼠骨骼肌细胞的实验研究
- Author:
Xinghua WANG
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Contrast media;
Muscle,skeletal;
Gene transfer techniques
- From:Chinese Journal of Ultrasonography
1993;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To explore the effect of SonoVue to gene transfer in skeletal muscle cells in mice.Methods H2K myoblast cells were treated by SonoVue and ultrasound (1 MHz,1 W/cm2) with pulse wave (20% duty cycle) using plasmid GFP (Green Fluorescent Protein) in vitro.Exposure duration was 10 s,20 s,30 s,40 s,50 s,and 60 s.The trend of transfection efficiency by flow cytometer and cell viability by Trypan blue assays were measured. In vivo studies,transfection efficiencies were assessed by counting GFP positive fibres on mice skeletal muscle.Tissue damage was assessed on serial sections.Results SonoVue did not enhance transfection efficiency compared with positive control in vitro,instead with increased cell killing rate. In vivo,SonoVue with or without ultrasound had significant effect on gene transfer without increased tissue damage.Conclusions Although SonoVue showed no effect in plasmid DNA transfection in skeletal muscle with reduced cell viability in vitro in this experimental condition,SonoVue had significantly enhanced gene transfection efficiency in mice in vivo without increased tissue damage.
