Advanced glycation end products-induced MCP-1 expression via its receptor RAGE in mouse podocytes
- VernacularTitle:糖基化终产物通过其受体诱导小鼠足细胞表达单核细胞趋化因子1
- Author:
Leyi GU
;
Zhaohui NI
;
Jiaqi QIAN
;
Tomino YASUHIKO
- Publication Type:Journal Article
- Keywords:
Podocytes;
Glycosylation end products, advanced;
Receptors, glycosylation end products, advanced;
Monocyte chemoattractant protein-1
- From:
Chinese Journal of Endocrinology and Metabolism
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate whether advanced glycation end products (AGE) induce monocyte chemoattractant protein-1 (MCP-1) expression in mouse podocytes in vitro and the role of AGE receptor (RAGE). MethodsThe effects of AGE, CML, S100 protein and neutralizing antibody against RAGE on MCP-1 gene and protein expressions were examined by RT-PCR and ELISA in mouse podocytes. ResultsRAGE was detected in undifferentiated and differentiated podocytes. AGE and CML dose-dependently stimulated MCP-1 expression in podocytes. At 8 h of incubation, AGE-and CML-treated podocytes produced more MCP-1 than that treated with bovine serum albumin (BSA), the MCP-1 concentrations being (7.44?1.01 and 8.06?0.96)ng/L for AGE and CML respectively vs (3.77?0.39)ng/L for BSA (both P
