Experimental Study :the Preparing of Targeted-directed Probe (Hab18-SPIO) and Testing of Its Phisichemical Characteristics
- VernacularTitle:Hab18-SPIO磁共振造影剂的制备及其实验研究
- Author:
Yanli LIU
;
Yi HUAN
;
Guangquan WEI
;
Yali GE
;
Yingjuan CHANG
- Publication Type:Journal Article
- Keywords:
MRI;
hepatocellular carcinoma(HCC);
superparamagnetic iron oxide particles(SPIO);
target-directed contrast agent
- From:
Journal of Practical Radiology
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare the Target-directed probe(Hab18-SPIO) and to test its phisichemical characteristics. Methods The McAb Target-directed probe(Hab18-SPIO) was prepared through hepatocellular carcinoma(HCC) McAb(Hab18) conjugated toSPIO using chemical method,while negative McAb probe(SED-SPIO) was also prepared using the same method.These agents weretested by high-performance liquid chromatography(HPLC) and electron microscope. Results The results of HPLC and electronmicroscope proved that Hab18 was conjugated SPIO effectively.The diameter of particles was between 50 and 70 nm.The binding rate of Hab18 was 97.5%. Conclusion The probe targeted to membrane-associated antigen of HCC(Hab18g) has been successfully prepared,which has novel physiochemical property.It is of great value in specific MR diagnosis of early HCCs.
