MRI in Evaluation of Transplantation of Autologous Marrow Stromal Cells Transfected Ex Vivo by VEGF Gene in a Porcine Chronic Myocardial Ischemia
- VernacularTitle:MRI评价VEGF转染自体骨髓基质细胞移植治疗慢性心肌缺血的实验研究
- Author:
Haiyun ZHU
;
Li WANG
;
Jianming TIAN
;
Yongde CHENG
;
Shengdong HUANG
;
Xunyu XU
- Publication Type:Journal Article
- Keywords:
myocardial ischemia;
marrow stromal cells;
vascular endothelial growth factor(VEGF);
cellular transplantation;
MRI
- From:
Journal of Practical Radiology
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the evaluation of multimodality MR imaging on the transplantation of autologous marrow stromal cells transfected ex vivo by vascular endothelial growth factor(VEGF)gene in a porcine chronic ischemic heart disease model. Methods Pigs of chronic ischemic heart disease model were randomly divided into two groups: treatment with marrow stromal cells transfected ex vivo by VEGF(Group I,n=9) and treatment with adenovirus served as control(Group Ⅱ,n=7).Four weeks after therapy,ejection fraction,ischemic segment and infracted segment were detected by multimodality MR imaging.The percentage of infarction area,CM-DiI labeled cells on fluorescence microscopy,vessel density were detected on specimen. Results In Group Ⅰ,in comparison with Group Ⅱ,the ejection fraction was significantly improved,the ischemic and infarction segments decreased(P
