Cross-match test of the donor-recipient of organ transplantation by ELISA
- VernacularTitle:酶联免疫吸附法在供、受者淋巴细胞毒交叉配合试验中的应用价值
- Author:
Qian FU
;
Wentao ZENG
;
Dong WANG
- Publication Type:Journal Article
- Keywords:
Enzyme-linked immunosorbent assay;
Antilymphocyte serum
- From:
Chinese Journal of Organ Transplantation
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the significance of enzyme-linked immunosorbent assay (ELISA) in cross-match test of donor-recipient before organ transplantation.Methods HLA glycoproteins were prepared by solubilizing the lymphocytes of donor with a non-ionic detergent, and bond to the monoclonal antibody specific for HLA class Ⅰ or Ⅱ immobilized in the ELISA tray. ELISA with addition of recipients' serum and complement-dependent cytotoxicity (CDC) were compared.Results Cross-match test was performed by two methods for lymphocytes of 40 donors and sera of 72 recipients. All samples were successfully tested. The results of one pair of donor-recipient cross-match test by two methods were different.Conclusion ELISA for cross-match test is simple, convenient and time-saving, but more sensitive and specific than CDC.
