Effects of interleukin-18 on transdifferentiation in renal tubular epithelial cells
- VernacularTitle:IL-18对肾小管上皮细胞表型转化的影响
- Author:
Cuiwei YAO
;
Dong LIANG
;
Huafeng LIU
;
Deshen TANG
;
Xiaowen CHEN
- Publication Type:Journal Article
- Keywords:
Interleukin-18;
Renal tubular epithelial cells;
Transdifferentiation;
?-Smooth muscle actin(?-SMA);
Transforming growth factor-?_1(TGF-?_1)
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of interleukin-18 on transdifferentiation in renal tubular epithelial cells(TECs).Methods:Human proximal tubular epithelial cell line(HK-2) was cultured in vitro.TECs were exposed to different concentrations(0,0.1,1,10 and 100 ng/ml) of IL-18 for 24,48 and 72 hours.At the end of each incubation,the expressions of the ?-SMA and TGF-?_1 mRNA were assessed by RT-PCR,the rate of ?-SMA expressing TECs was assessed by immunocytochemistry,and the expression of the ?-SMA protein was assessed by Western blotting.Results:(1)The expressions of ?-SMA and TGF-?_1 mRNA were increased significantly by a dose- and time-dependent manner when TECs were exposed to IL-18(P
