Expression of endogenous erythropoietin of rat neural stem cells after hypoxia
- VernacularTitle:大鼠神经干细胞缺氧后的内源性促红细胞生成素表达规律
- Author:
Chongyi SUN
;
Meng YAO
;
Limin CHEN
- Publication Type:Journal Article
- Keywords:
Hypoxia;
Preconditioning;
Stem cells;
Erythropoietin
- From:
Orthopedic Journal of China
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the endogenous erythropoietin expression of neural stem cells(NSCs) of rats after hypoxia.[Method]We used a rat NSCs culture model.EPO expression after 4 hours of hypoxia was studied.It was examined again following 12 hours of hypoxic lethal insult which was applied various times after hypoxic preconditioning.EPO expression was studied by western blotting.[Result]Following the preconditioning,expression of EPO increased immediately and reached a peak 4 hours later.It lasted till 8 hours later.While applied the lethal hypoxia,EPO expression of cells in preconditioning 24 hours group were was significantly increased than that in preconditioning 4 hours group.[Conclusion]The hypoxic preconditioning can lead an increased endogenous EPO expression and decrease the threshold of EPO expression against subsequent lethal stimulation during a certain period.
