ULTRAVIOLET ABSORPTION OF BILE ACIDS IN MINIBECK REAGENT
- VernacularTitle:胆汁酸在Minibeck试剂中的紫外吸收
- Author:
Jianxin LI
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The characteristic ultraviolet absorption of ten kinds of free and conjugated bile acids in Minibeck reagent is observed. The monohydroxycholanic acids have absorption maximum at 310 nm, dihydroxycholanic acids a strong maximum at 310 nm and a weaker maximum at 390 nm,but trihydroxycholanic acids a strong maximum at 390 nm and a weaker maximum at 310 nm. The molar absorptivities of free and conjugated bile acids in one type are approximately equal under 310 or 390nm. The absorbance is in linear proportion to concentration of bile acids in Minibeck reagent below 5-10 mM, and it is stable at least for 48 hours under cold and dark storage. A trihydroxycholanic acid solution containing mono-or dihydroxy-cholanic acid maintains their original absorption feature, and has apeak value of additive property.Each component of bile acids could be calculated by the absorbance at 310 and 390 nm.
