Effect of NS398 on anti-proliferation and inducing apoptosis of human osteosarcoma cell MG-63 line
- VernacularTitle:NS398对人骨肉瘤细胞MG-63增殖与凋亡的影响
- Author:
Eryou FENG
;
Renyun XIA
;
Yiyuan SHI
;
Yanbin LIN
- Publication Type:Journal Article
- Keywords:
cyclooxygenase-2;
cultured tumor cells;
apoptosis
- From:
China Oncology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Background and Purpose:Cyclooxygenase-2(COX-2) plays an important part in tumor genesis,growth,and angiogenesis.Many inhibitors of COX-2 could inhibit proliferation and induce apoptosis of cancer cells. This study investigated the impact of NS398 on anti-proliferation and the induction of apoptosis in human osteosarcoma cell line MG-63.Methods:Cell proliferation is measured by MTT method.Characteristic changes of apoptosis in morphology are observed by fluorescence microscopy、transmission electron microscopy(TEM) and quantitatively by TDT-mediated dUTP-biotin nick end-labeling(TUNEL) assay.The apoptotic rates are calculated by flow cytometry(FCM).Results:The growth inhibition rates of MG-63 cells treated with 1,10,50,100 and 200 ?mol/L NS398 are 14.7%,23.5%,33.6%,52.5% and 81.4%,respectively(P
