Analysis of chromosome variation of lymphocytes and comparative genomic hybridization of lung carcinoma in patients with long survival after operation
- VernacularTitle:高生存期肺癌患者外周淋巴细胞染色体及肺癌组织比较基因组杂交回顾性分析
- Author:
Wei XIONG
;
Guisheng QIAN
- Publication Type:Journal Article
- Keywords:
lung carcinoma;
chromosome;
comparative genomic hybridization
- From:
China Oncology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Background and Purpose:To analyse the chromosome of peripheral lymphocytes and tissue samples of lung cancer for the long term survival patients after surgery,which could provide the rationale for the screening of relative genes of carcinogenesis and clinical treatment of lung cancer.Methods:Twenty patients with over 5 year survival after operation were defined as long term survival group.There were 9 patients with squamous cell carcinoma,6 with adenocarcinoma and 5 with small cell carcinoma in the long term survival group.The control group was defined as the samples from the patients with lung cancer who had surgery within one year and had to be randomly chosen,8 patients were histologically proven as squamous cell carcinoma,7 as adenocarcinoma and 5 as small cell carcinoma in the control group.The samples from healthy donors were defined as the normal group.The chromosome variations of peripheral lymphocytes in all these 3 groups were analyzed by routine methods.The comparative genomic hybridization of the embed-paraffin tissue sample of lung carcinoma was done by the DeVries methods.Results:In the long term survival group,the chromosome variations rate of lymphocytes was 3.15%,which was lower than that of the control group(3.15% vs 10.85%,P
