Chemotaxis on Umbilical Vein Endothelial Cells of SMMC7721 Hepatic Carcinoma Cells with Angiopoietin Gene Expression
- VernacularTitle:携带促血管生成素基因的肝癌细胞对血管内皮细胞的趋化作用
- Author:
Lin PENG
;
Jinrui OU
;
Jian SUN
- Publication Type:Journal Article
- Keywords:
Angiopoietin Hepatic carcinoma cell line Chemotaxis
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the chemotactic role on umbilical vein endothelial cells of SMMC7721 hepatic(carcinoma) cells with angiopoietin gene expression in order to study the effects of angiopoietin on hepatocellular carcinoma angiogenesis.Methods Angiopoietin gene 1(Ang-1) fragment and Ang-2 fragment was transfected into SMMC7721 liver carcinoma cell line by Lipofectamine induced gene transfection technique.The chemotactic role of SMMC7721 liver carcinoma cell line on umbilical vein endothelial cells was observed through microchemotaxis analysis.Results The chemotactic response of the Umbilical vein endothelial cells was obviously improved with Ang-1 expression (P0.05).Conclusion Ang-1 is a chemotactic factor for vascular endothelial cell and a promoter for angiogenesis,whereas Ang2 does not show obvious chemotactic role.
