Study of E-cadherin Antisense Oligodeoxynucleotide on Invasive Ability of Tumor Cells
- VernacularTitle:E-cadherin反义寡核苷酸对肿瘤细胞浸润能力影响的研究
- Author:
Xiangming CHE
;
Shufeng WANG
;
Lin FAN
;
Ruyuan ZHANG
;
Wei ZHAO
- Publication Type:Journal Article
- Keywords:
E-cadherin Tumor cell Invasion Antisense oligodeoxynucleotied
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of down-regulation of E-cadherin on the invasion ability of tumor cells.Methods Human pancreatic carcinoma cell line JHP-1 was treated with E-cadherin antisense oligodeoxynucleotied(ASODN).The immunocytochemistry,Western blot were used to detect the expression and the contents of(E-cadherin) in the tumor cells,and the invasive ability of tumor cells were evaluated by invasive-MTT assay.Results Treated with E-cadherin ASODN,the expression of E-cadherin on JHP-1 cells were reduced,and the protein contents were decreased as well compared with control groups and ODN group.The invasive ability of JHP-1 cells to the basement membrane was increased(P
