Effects of U0126 on brain edema and aquaporin 4 expression in rat brains after cerebral ischemic injury
- VernacularTitle:U0126对缺血性脑损伤大鼠脑内水通道蛋白-4表达的影响
- Author:
Dengjun GUO
;
Yinghui CHEN
;
Yongbo ZHAO
;
Naidong WANG
- Publication Type:Journal Article
- Keywords:
Brain ischemia;
Brain edema;
Aquaporins;
Butadienes;
Nitriles
- From:
Chinese Journal of Neurology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of U0126 on brain edema and the expression of aquaporin 4 (AQP4) in rat brains after cerebral ischemic injury.Methods Totally 48 healthy male SD rats were divided randomly into ischemia group, treatment group and normal group.Rats in ischemia group and treatment group underwent middle cerebral artery occlusion by using an intraluminal thread method.Thirty minutes before operation, the rats in treatment group were injected into lateral cerebral ventricle with U0126, while rats in ischemia group accepted normal saline.24 hours after operation, the water content and Evans Blue in rat brains were determined as to exploring the degree of brain edema.Immunohistochemistry,Western blot and RT-PCR technique were applied to detect AQP4, p-ERK1/2 and p-ELK1.Results Compared with normal group, the water content and AQP4 expression in ischemia group were increased obviously.The water content and AQP4 expression in treatment group (protein:149.0?1.1,mRNA:0.328?0.010) were lower than those in ischemia group (protein:153.6?0.8,mRNA:0.400?0.015,P
