Detection of Potential Microcystin-Producing Cyanobacteria in Waters With a mcyD Molecular Biomarker
- VernacularTitle:水体中蓝绿藻分子标志物——mcyD基因的检测
- Author:
Jiliang SI
;
Wenrong HU
;
Jie LI
- Publication Type:Journal Article
- Keywords:
Water pollution;
Algae;
Gene;
Microcystins
- From:
Journal of Environment and Health
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a new method to screen the potential microcystin-producing cyanobacteria in natural waters. Methods Cyanobacteria were cultured and waters samples were analyzed by polymerase chain reaction with special primers for gene of the operon mcyD which encodes a microcystin synthetase combining conserved gene 16S rRNA of alga, subsequently, whose microcystins were detected by ELISA. Results There was a unique amplified product of approximately 870 bp in microcystin-producing blue-green alga and water samples, but no target band in non-toxic strains and samples. Conclusion It is feasible to use the molecular biological method used mcyD as the microcystin molecular biomarker to screen microcystin-producing cyanobacteria in natural water.
