Study on Formaldehyde-Induced DNA Damage in Mice Testicular Cells
- VernacularTitle:甲醛对小鼠睾丸细胞DNA的损伤作用
- Author:
Xiaoping WANG
;
Liju DUAN
;
Chenlan LI
- Publication Type:Journal Article
- Keywords:
Formaldehyde;
DNA damage;
Comet assay;
Reproductive toxicity
- From:
Journal of Environment and Health
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To exlplore the adverse effect of formaldehyde on DNA in isolated mice testicular cells. Methods The testicular cells were exposed to formaldehyde of different concentrations(0, 25, 50, 75, 100 ?mol/L), comet assay was applied to detect DNA breaks. Results Formaldehyde induced DNA breaks at 10 ?mol/L, 25 ?mol/L and 50 ?mol/L (P0.05). The critical concentration at which formaldehyde could induce DNA strand breaks of testicular cells was about 10 ?mol/L, peak point was near 50 ?mol/L. Conclusion Formaldehyde may cause DNA damage in isolated mice testicular cells in various degree.
