Inhibitory effects of Th2 cytokines and anti-IL-12R?1 mAbs on IL-23 inducing IFN-? production by normal human PBMCs
- VernacularTitle:Th2细胞因子和抗IL-12R?1mAb抑制由IL-23诱导PBMCIFN-?的产生
- Author:
Yanying FAN
;
Changyou WU
- Publication Type:Journal Article
- Keywords:
IL-23;
IFN-?;
Th2 cytokines;
Anti-IL-12?1 mAb;
NK cells
- From:
Chinese Journal of Immunology
1985;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the role of IL-23 in the production of IFN-?, cell subsets and regulation by human peripheral blood mononuclear cells(PBMCs).Methods:PBMCs were isolated from normal human peripheral blood and cultured with IL-23 in different culture conditions. The level of IFN-? in the culture supernatants was examined by ELISA. The subsets and frequency of IFN-?-producing cells were examined at a single cell level by flow cytometry.Results:IL-23 could directly induce IFN-? production by PBMCs and have synergistic effect with IL-2 on the induction of IFN-? production in a dose dependent manner. The data from Flow cytometric analysis indicated that IL-23 could induce IFN-? expression by CD3~-CD56~+NK cells but not CD3~+CD4~+ and CD8~+T cells. It is noted that IL-23 predominantly induced IFN-? expression by NK cells with high expression of CD56 molecules. Addition of Th2 cytokines or anti-IL-12R?1 mAbs resulted in the inhibition of IL-23-inducing IFN-? production.Conclusion:IL-23 can directly induce IFN-? production by PBMCs. The induction of IFN-? induced by IL-23 can be suppressed by Th2 cytokines and anti-IL-12R?1 mAbs. The data indicated that Th2 cytokines and anti-IL-12R?1 mAbs might have the potential application for the treatment of IL-23-mediated autoimmune diseases.
