A quick way in vitro for amplification of goat chondrocytes by RCSS
- VernacularTitle:羊软骨细胞在生物反应器中的培养和扩增
- Author:
Li ZHANG
;
Quanyi GUO
;
Xiang SUI
- Publication Type:Journal Article
- Keywords:
Cartilage;
Tissue engineering;
Microcarrier;
Cell culture
- From:
Orthopedic Journal of China
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To establish a method for obtaining well-differentiated goat chondrocytes quickly in large scale by RCSS using microcarriers technique. [Method]Articular chondrocytes were harvested from goat by sequential digestion with trypsin and collagenase , and then grown in RCSS bioreactor culture system which contained the Cytodex-3 microcarriers in the culture medium (DMEM) . Growth of chondrocytes on Cytodex-3 microcarriers was observed dynamically under phase contrast microscope . Immunocytochemical analysis was performed for type Ⅰcollagen and type Ⅱcollagen. [Result]The articular chondrocytes attached rapidly to the surface of Cytodex-3 microcarriers. Quick growth of these cells was observed after they fully spread onto the microcarriers. The density of chondrocytes increased by 15～17 times in later stage of culture as compared with the initial density. The harvested chondrocytes had no detectable staining for collagen type Ⅰ, but stained intensively for collagen type Ⅱ. [Conclusion]Microcarrier culture of chondrocytes can yield a large quantity of goat cells within a short time ,which will be of benefit for banking cartilage cells for reconstruction of impaired cartilage by way of tissue engineering.
