Effects of hypoxia on apoptosis of human lung adenocarcinoma A549 cell line by adriamycin
- VernacularTitle:乏氧对多柔比星诱导肺腺癌A549细胞凋亡的影响
- Author:
Shu XIA
;
Shiying YU
- Publication Type:Journal Article
- Keywords:
lung neoplasms;
hypoxia inducible factor-1?;
P-glycoprotein;
apoptosis
- From:
China Oncology
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:Hypoxic cells in tumors are of chemoresistant and anti-apoptoic.Our study was focused on the effects of hypoxia on expression of P-glycoprotein(P-gp) and cell apoptosis in human lung adenocarcinoma A549 cell line(wtP53),in order to explore the probable mechanism of hypoxia in apoptic tumor cells.Methods:A549 cell line(human lung adenocarcinoma) was cultured under hypoxic atmosphere(2% O_2) for 24 h,the expression of hypoxia inducible factor-1?、P-gp and P53 protein was detected by immunohistochemistry,after cells under hypoxic atmosphere were exposed to Adriamycin for 24 h,the cell survival rate was detected by MTT,and apoptosis of by flow cytometer.Results:① The expression of hypoxia inducible factor-1?、P-gp and P53 protein in hypoxic cells were higher than the expression in anoxia ,and correlation between the expression of HIF-1? and P-gp or P53 protein was observed(P
