Association of protein tyrosine phosphatase-1B gene polymorphism with type 2 diabetes mellitus and obesity in Chinese
- VernacularTitle:蛋白酪氨酸磷酸酶1B基因多态性与2型糖尿病和肥胖的相关性研究
- Author:
Hongli ZHANG
;
Hongda ZHU
;
Tianhong LUO
;
Qinqin WANG
;
Lizhen SU
;
Luo LU
;
Liebin ZHAO
;
Meng DAI
;
Youping LIU
;
Jiping LI
;
Jian YANG
;
Yun LIU
;
Ling JIANG
;
Guo LI
;
Min LUO
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus, type 2;
Obesity;
Protein tyrosine phosphatase-1B gene;
Polymorphism, single nucleotide;
Waist-hip ratio
- From:
Chinese Journal of Endocrinology and Metabolism
1985;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the association of single nucleotide polymorphism (SNP) of the protein tyrosine phosphatase-1B (PTP-1B) gene with type 2 diabetes mellitus and obesity in Chinese. Methods SNPs in the PTP-1B gene were detected by direct sequencing to PCR products, and the detected SNPs were genotyped in case-spouse samples with the technique of fluorescence real-time PCR. Results Totally 6 SNPs were found in PTP-1B gene. Three SNPs (I5/37 C→A,I6/82 A→G, I7/301 C→T) were in the intron regions and the other 3 (E8/45 C→T, E9/35 G→A, E10/372 G→A)in the exon regions. Among them, E9/35 G→A was a newly found mutation site. The A allele frequency of I5/37 C→A, T allele frequency of I7/301 C→T and G allele frequency of I6/82 A→G in type 2 diabetes were significantly higher than those in the normal spouse group (P
