Detection of Borna disease virus p24 in cerebrospinal fluid and peripheral blood cells in patients with viral encephalitis
- VernacularTitle:病毒性脑炎患者血液和脑脊液博尔纳病病毒p24的检测
- Author:
Zhenhai WANG
;
Peng XIE
;
Ping YANG
;
Fanyuan KONG
- Publication Type:Journal Article
- Keywords:
Encephalitis,viral;
Borna disease virus;
Viral proteins;
Reverse transcriptase polymerase chain reaction
- From:
Chinese Journal of Neurology
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to investigate the relationship between Borna disease virus(BDV) and the viral encephalitis,and whether BDV infection exists in Ningxia or not.Methods The p24 fragment of BDV in peripheral blood mononuclear cells(PBMC) and cerebrospinal fluid mononuclear cells(CSFMC) in 52 patients with viral encephalitis and 32 healthy donors were examined by nested reverse transcriptase polymerase chain reaction(RT-PCR) and fluorescence quantitative(FQ) PCR to analysis gene sequence,homogenicity and amino acid sequence of positive products.Results The positive rate of BDV p24 in PBMC and CSFMC in viral encephalitis(9.6%,11.5%) was significantly higher than that in healthy donors(0,P
