Prolongation of renal allografts survival in rats by tolerogenic recipient dendritic cells loaded with donor renal antigen
- VernacularTitle:负载供肾抗原的致耐受性树突状细胞延长大鼠移植肾存活时间
- Author:
Chibing HUANG
;
Yinfu ZHANG
;
Minqi FAN
- Publication Type:Journal Article
- Keywords:
Dendritic cells;
Antigen;
Kidney transplantation
- From:
Chinese Journal of Organ Transplantation
1996;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of recipient tolerogenic dendritic cells(DCs) loaded with donor renal antigen on renal allografts survival in rats.Methods Bone marrow derived DCs of Lewis rats were loaded with antigens from donor kidney of BN rats and transferred with AdvCTLA-4Ig to generate tolerogenic DCs.The tolerogenic DCs were injected into the Lewis recipient 24 h before BN→Lewis kidney transplantation(treatment group).Survival time of renal allografts was observed and splenic cell reaction of the recipients to donors and the third party antigens were assayed by means of MTT on the 20~(th)postoperative day.Results Survival time of renal allografts in treatment group was prolonged significantly as compared with controls ((62.1)?(13.7) days vs(8.6)?(0.9) days,P
