Effects of celecoxib on the apoptosis in acute cardiac allograft rejection in rats
- VernacularTitle:塞来昔布对大鼠心脏移植急性排斥反应的抑制作用
- Author:
Guangquan XU
;
Bin WANG
;
Xiangyuan JIN
- Publication Type:Journal Article
- Keywords:
Heart transplantation;
Celecoxib;
Cell death;
Graft rejection
- From:
Chinese Journal of Organ Transplantation
1996;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of celecoxib on apoptosis during acute rejection of cardiac allograft in rats.Methods Forty Wistar rats underwent heterotopic heart transplantation in the abdominal portion from disparate SD rats.The cardiac grafts were harvested at 3rd and 5th day after transplantation.The cardiac allograft sections were subjected to the HE staining and in situ terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling(TUN EL) for histopathological rejection grade and apoptotic index(AI).Results Apoptosis mainly occurred in cardiac myocytes throughout allograft rejection.Celecoxib could markedly reduce myocyte apoptosis and abate the damage to the graft tissue.The AI at 3rn and 5th day posttransplantation were(2.35)?(1.51) and(11.35)?(3.46) in control group,and (1.03)?(0.42) and(3.28)?(2.42) in celecoxib group respectively with the difference between them being significant.Conclusions Apoptosis is an important mechanism of cell death in acute cardiac allograft rejection in rats.Celecoxib can apparently inhibit the apoptosis of cardiac myocytes,which may be an important way of its immunosuppressive actions.
