Cultivation of human bone marrow mesenchymal stem cells and induced differentiation into neuron-like cells
- VernacularTitle:成人骨髓间充质干细胞的培养及其向神经元样细胞诱导分化的实验研究
- Author:
Gang CHEN
;
Shangzhen QIN
;
Lianting MA
- Publication Type:Journal Article
- Keywords:
Bone marrow;
Mesenchymal stem cells;
Cell Culture techniques;
Neurons
- From:
Chinese Journal of Organ Transplantation
1996;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore a way that induce human bone marrow mesenchymal stem cells to differentiate into neuron-like cells in vitro.Methods Bone marrow mesenchymal stem cells were cultivated in a medium containing serum by using density gradient centrifugation method and whole marrow method and were induced to differentiation by ?mercaptoethanol.Immunohistochemistry were used to identify the type of cells.Results The number of cells was((1.73)?(0.44))?10~(7)/ml by using whole marrow method and ((7.65)?(0.52))?10~(7)/ml by using density gradient centrifugation method at the 10th day cultivation respectively.There was statistically significant difference(P
