Adult human bone marrow-derived mesenchymal stem cells suppresses allogeneic lymphocytes proliferation
- VernacularTitle:成人骨髓间充质干细胞抑制异体淋巴细胞增殖的实验研究
- Author:
Tao WU
;
Hai BAI
;
Cunbang WANG
- Publication Type:Journal Article
- Keywords:
Bone marrow;
Mesenchymal stem cells;
Cell Culture techniques;
Lymphocytes
- From:
Chinese Journal of Organ Transplantation
1996;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the immunomodulatory functions of adult human bone marrow-derived mesenchymal stem cells in vitro.Methods Adult human BMMSCs were separated with Percoll((1.073) g/ml) and cultured in Dulbecco's Modified Eagle's Medium with low glucose containing 10% fetal calf serum.The purity of BMMSCs was identified with the spindle-fibroblastic morphology by microphotograph and the phenotypes were tested by fluorescence-activated cell sorter(FACS).BMMSCs were plated in 96-well plates.After treated with mitomycin,BMMSCs were co-cultured for 72 h with allogeneic lymphocytes isolated from peripheral blood.Effects of MSCs on PHA induced lymphocytes transformation were investigated.The proliferation of lymphocytes was measured by MTT method.Results Peripheral blood lymphocytes proliferation was suppressed by BMMSCs and the inhibition ratio was (60.68%)(P
