Effect of EC-PAOA on the Proliferation of Eutopic Endometrial Cells in Vitro.
- VernacularTitle:玉竹提取物C对子宫内膜异位症在位细胞增生的作用
- Author:
Yanfeng YANG
- Publication Type:Journal Article
- Keywords:
EC-PAOA;
Endometriosis;
Separated cells;
MTT
- From:
Journal of Medical Research
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Here we intend to find out activity of EC-PAOA on the proliferation of normal endometrial and eutopic endometrial cells in vitro.Methods Normal human endometrium and eutopic human endometriosis were used,stromal and epihelisl cells were separated by selective enzymatic digestion method,Cells types and purities of cells were identified through immunohistochemistry result;The isolated cells were primarily cultured,while the other pat cultured with different concentrations of EC-PAOA(10,100,1000ug/ml ) for 24,48 and 72h,The effect of cultured cells from nomal endometrium and eutopic endometriosis were observed by MTT assay.Results EC-PAOA was observed no effect on normal endometrium, in the contrast, EC-PAOA was found to anti proliferation of eutopic endometrial cells and was most notable to anti proliferation of eutopic endometrial cells(P