Synergistic effect of anti-human DR5 monoclonal antibody and adriamycin on SMMC-7721 cells
- VernacularTitle:抗人DR5单抗与阿霉素联合对人肝癌细胞SMMC-7721协同杀伤效应
- Author:
Jun ZHANG
;
Yuanfang MA
;
Guangchao LIU
;
Shulian LI
;
Feng LU
- Publication Type:Journal Article
- Keywords:
Anti-human DR5 monoclonal antibody(mDRA6);
Adriamycin;
Synergistic effect;
Apoptosis
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the sensitivity of SMMC-7721 cells to mDRA-6,the synergistic damage effect of mDRA-6 and adriamycin on human hepatocellular carcimoma cell lines and its possible mechanism.Methods:SMMC-7721 cells were cultured with RPMI1640 medium in regular condition.The morphology was observed by microscope.Cytotoxicity was examined by MTT assay. Apoptosis were detected by flow cytometry.Results:(1)The apoptosis of SMMC-7721 cells could be induced by mDRA-6,1.89 ?g/ml of mDRA-6 cound kill 36% of the cells;(2)Concentration-dependent cytotoxicity of adriamycin was exhibited in SMMC-7721 cells;(3)The combination of mDRA-6 and adriamycin exhibited synergistic effect on SMMC-7721 cells.2 ?g/ml of mDRA6 and 40 ng/ml of adriamycin killed 60%SMMC-7721.Conclusion:MDRA-6 can induce SMMC-7721 cell apoptosis.The combination of mDRA-6 and adriamycin exhibit synergistic effect on SMMC-7721 cells.
