Inhibitory effects of muscone on PMNs adherence to HUVEC and the expression of ICAM-1,VCAM-1 and CD44 of HUVEC
- VernacularTitle:麝香酮抑制血管内皮细胞与中性粒细胞黏附及其表面ICAM-1、VCAM-1和CD44表达
- Author:
Xiujuan HE
;
Ping LI
;
Quanying QIU
;
Xun SHENG
;
Fang WANG
;
Jinli LOU
- Publication Type:Journal Article
- Keywords:
HUVEC;
PMN;
Adhesion;
Adhesion molecules;
Wound healing
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Based on the model of human umbilical vein endothelial cell(HUVEC) treated by tumor necrosis factor-alfa(TNF),We investigate the effects of muscone on polymophonulear leukocytes(PMN)-HUVEC adhesion and its adhesion molecules(CAMs) expression.Methods:Confocal system was used for identifying cultured HUVEC,MTT assay for its activity,Rose Bengal Staining for PMN-HUVEC adhesion,and fluorescent-immunocytochemistry techniques for CAMs expression.Results:After HUVEC treated by TNF,the adhesion between PMN and HUVEC increased dramatically(P
