Isolation,purification and identification of human periodontal ligament stem cells
- VernacularTitle:人牙周膜干细胞的体外分离、纯化及初步鉴定
- Author:
Qin GAO
;
Hongwei LIU
;
Yan JIN
- Publication Type:Journal Article
- Keywords:
Periodontal ligament stem cell;
Cell cloning;
Ultrastructure;
Cell cycle;
Immunohistochemistry
- From:
Journal of Practical Stomatology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To isolate and identify the periodontal ligament stem cells.Methods:Periodontal ligament tissues were digested with a mixture of collagenase and Dispase,then the periodontal ligament stem cell clones were isolated by limited dilution method.The transmission electron microscopy(TEM),flow cytometery and immunohistochemistry procedure were employed to study the ultrastructure,cell cycle and surface marker of the cloned cells.Results:The obtained cells showed the characteristics of undifferentiated morphology at ultrastructural level.94.4% of the cells were in phase G_0/G_1.The cells were Vimentin,STRO-1,ON and BSP positive.Conclusion:Cloning incubation may be the effective way to isolate and purify the periodontal ligament stem cells.
