The effect of TGF-? on osteoclast differentiation in vitro
- VernacularTitle:转化生长因子?对体外破骨细胞分化的影响
- Author:
Yuming ZHAO
;
Lihong GE
;
Ae GRIGORIADIS
- Publication Type:Journal Article
- Keywords:
Receptor activator of NF-?B ligand;
Macrophage colony stimulating factor;
Transforming growth factor-?;
Osteoclast;
Bone marrow cells
- From:
Journal of Practical Stomatology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of TGF-? on osteoclast differentiation in primary murine bone marrow cell culture in the presence of receptor activator of NF-?B Ligand(RANKL) and macrophage colony stimulating facotr(M-CSF).Methods:M-CSF-dependent bone marrow cells were isolated from 5~6 weeks old mice,and cultured in the presence of RANKL at 30 ng/ml and M-CSF at 25 ng/ml with or without 1 ng/ml of TGF-? for 4 and 9 days respectively.TRAP positive multinucleated cells and resorption pits on dentine slices were counted under light microscope.Results:TRAP positive multinucleated cells were induced from murine bone marrow cell cultures with both RANKL and M-CSF,and these cells formed resorption pits on dentine slices.The number of TRAP positive multinucleated cells and resorption pits were significantly increased by 1 ng/ml of TGF-?(P
