Effects of IGF-1 on the biological characteristics of mouse dental follicle cells
- VernacularTitle:IGF-1对小鼠牙囊细胞生物学特性的影响
- Author:
Shaohua GE
;
Pishan YANG
;
Shu LI
- Publication Type:Journal Article
- Keywords:
Dental follicle cells;
Cytodifferentiation;
IGF-1
- From:
Journal of Practical Stomatology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of IGF-1 on the proliferation,total protein amount and cytodifferentiation of mouse dental follicle cells.Methods:The dental follicle cells of passage 3 were incubated with different concentrations((0.005),0.01,0.05,0.1 and 0.5 mg/L)of IGF-1 respectively,cell proliferation,alkaline phosphatase(ALP),total protein amount were measured by MTT assay and spectrophotometer respectively.Effects of 0.05 and 0.1 mg/L IGF-1 on mineralization potential were studied by Von Kossa staining.Results:IGF-1(ml/L) at 0.005~0.1 increased the proliferation and total protein amount(P
