The effect of gtfB specific antisense oligodeoxyribonucleotides on S.mutans sucrose-dependent adherence to saliva coated hydroxyapatite in vitro
- VernacularTitle:体外观察反义脱氧寡核苷酸对变形链球菌蔗糖依赖性粘附的影响
- Author:
Qingyu GUO
;
Junzheng WU
;
Gang XIAO
- Publication Type:Journal Article
- Keywords:
Caries;
Streptococcus mutans;
Antisense oligodeoxyribonucleotide;
Sucrose-dependent adherence
- From:
Journal of Practical Stomatology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of gtfB specific antisense phosphorothioate-modified oligodeoxyribonucleotides(PS-ODNs) on S.mutans sucrose-dependent adherence.Methods:Antisense oligodeoxyribonucleotide targeted to gtfB sequence 709~726 bp(PS-ODN1) and 3 479~3 497 bp(PS-ODN2) were synthesized.Natural genetic transformation of S.mutans with PS-ODN1 and PS-ODN2 was respectively performed.Adhesion of S.mutans to saliva coated hydroxyapatite was examined by crystal violet staining and destain with absolute ethanol.The absorbance at 620 nm was measured by plate reader(the absorbance value derived from the wells without sucrose was used as background and was subtracted).Results:The adhesion ability of the strains treated with antisense PS-ODN was significantly lower than that of the control(P
