Effects of roxithromycin on TNF-? and IL-6 release from periodontal ligment fibroblasts stimulated with lipopolysaccharide
- VernacularTitle:罗红霉素对人牙周膜成纤维细胞TNF-?、IL-6释放的影响
- Author:
Yongxiang FAN
;
Qi LIU
;
Shaowei ZHANG
- Publication Type:Journal Article
- Keywords:
Periodontitis;
Roxithromycin;
Lipopolysaccharide;
Periodontal ligament fibroblasts;
Tumor necrosis factor-alpha;
Interleukin-6
- From:
Journal of Practical Stomatology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To test the effects of roxithromycin(RM) on TNF? and IL-6 release from periodontal ligment fibroblasts(PDLCs) stimulated with lipopolysaccharide(LPS).Methods:PDLCs of passage 4~8 were cultured with RM at 2,20 and 200 ?g/ml respectively with 10 ?g/ml of LPS.The control cells were cultured with culture medium only.The cultures were continued for 3,6,12 and 24 hours respectively.ELISA method was used to measure TNF-? and IL-6 released into the culture medium from PDLCs in the different groups.Results:LPS increased both TNF-? and IL-6 release from PDLCs at all the time points(P
