Ultrasound-mediated PTEN gene inhibit invasion and metastasis of human ovarian carcinoma SKOV3 cell lines
- VernacularTitle:超声造影剂介导PTEN基因影响卵巢癌侵袭的实验研究
- Author:
Bin WANG
;
Yibing FU
;
Xiaocui LI
;
Li SUN
;
Qingzhao FU
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Contrast media;
Ovarian neoplasms;
Gene therapy
- From:
Chinese Journal of Ultrasonography
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the transfection of PTEN gene in human ovarian carcinoma SKOV3 cells by ultrasound mediating and contrast agent′s enhancing, and to explore the mechanism of PTEN gene inhibiting the tumor cells′ invasion. Methods After addition of PTEN gene and 5 ?l lipofectamine,human ovarian carcinoma SKOV3 cells on 6-well plates were explored to ultrasound for 60 s in the presence(200 ?l) of ultrasound agent. The invasiveness of transfected cells was measured quantitatively by Matrigel invasion assays(Transwell chamber) after 48 h. Results The invasiveness of SKOV3 cells transfected by ultrasound-mediated PTEN gene was significantly declined compared with that of the controls. Conclusions The transfection of PTEN gene into SKOV3 cell can inhibit their invasion.The gene transfection can be enhanced by ultrasound and contrast agent,which may be expected to be a new means of clinical gene therapy.
