Effect of Metformin on Cell Growth and Differentiation in Cultured Odontoblasts.
10.11620/IJOB.2017.42.2.039
- Author:
Chang Young OH
1
;
Su Gwan KIM
;
Dae San GO
;
Sun Kyoung YU
;
Tae Hoon KIM
;
Chun Sung KIM
;
Joo Cheol PARK
;
Do Kyung KIM
Author Information
1. Oral Biology Research Institute, School of Dentistry, Chosun University, Gwangju 61452, Republic of Korea. kdk@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
metformin;
odontoblasts;
differentiation;
mineralization
- MeSH:
Animals;
Cell Differentiation;
Cell Proliferation;
Dental Papilla;
Humans;
Metformin*;
Mice;
Miners;
Odontoblasts*;
RNA, Messenger
- From:International Journal of Oral Biology
2017;42(2):39-45
- CountryRepublic of Korea
- Language:English
-
Abstract:
Metformin (1,1-dimethylbiguanide hydrochloride), derived from French lilac (Galega officinalis), is a first-line anti-diabetic drug prescribed for patients with type 2 diabetes. However, the role of metformin in odontoblastic cell differentiation is still unclear. This study therefore undertook to examine the effect of metformin on regulating odontoblast differentiation in MDPC-23 mouse odontoblastic cells derived from mouse dental papilla cells. As compared to controls, metformin significantly accelerated the mineralization, significantly increased and accelerated the expressions of ALP and Col I mRNAs, and significantly increased the accelerated expressions of DSPP and DMP-1 mRNAs, during differentiation of MDPC-23 cells. There was no alteration in cell proliferation of MDPC-23 cells, on exposure to metformin. These results suggest that the effect of metformin on MDPC-23 mouse odontoblastic cells derived from mouse dental papilla cells, facilitates the odontoblast differentiation and mineralization, without altering the cell proliferation.
