Study on the Toxicity of Ambient PM2.5 to Primary Cultured Myocardial Cells of Neonatal Rats
- VernacularTitle:大气PM_(2.5)对原代培养大鼠心肌细胞的毒性
- Author:
Canjun ZHENG
;
Feifei WANG
;
Xinbiao GUO
- Publication Type:Journal Article
- Keywords:
Air pollution;
Particulate matter;
Myocardial cell
- From:
Journal of Environment and Health
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the ambient PM2.5 component and the toxicity to primary cultured myocardial cells of neonatal rats. Methods Primary myocardial cells cultures were prepared from 1-day-old Sprague-Dawley rats and treated with PM2.5 suspension and its organic and water-soluble extracts at various concentrations for 24 h. The cellular viability was measured with MTT methods, and the beat of myocardial cells was observed and counted under inverted microscope. Results PM2.5 suspension and its organic and water-soluble extracts increased the viability of myocardial cells at the concentrations below 10.0 ?g/ml, but above this, they could significantly decrease the viability of myocardial cells with a dose-dependent manner. The toxicity of organic extract of PM2.5 was significantly higher than that of water-soluble extract. PM2.5 suspension and its organic and water-soluble extracts also dose-dependently inhibited the beat frequencies of myocardial cells. Conclusion PM2.5 and its extracts show a hormesis effect on the cultured myocardial cells in the case of the cellular viability is taken as the response parameter. PM2.5 and its extracts can inhibit the beat of myocardial cells.
