Cloning of SARS Sc gene and its effect on the immune response of mouse elicted by DNA vaccine pcDNA-Sc
- VernacularTitle:SARS冠状病毒S蛋白C端片段基因的克隆及其DNA疫苗的免疫学研究
- Author:
Xianfen YUAN
;
Shanye YIN
;
Hong DING
- Publication Type:Journal Article
- Keywords:
DNA vaccine;
Severe acute respiratory syndrome;
Coronavirus;
Spike protein
- From:
Chinese Journal of Immunology
1985;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To construct DNA vaccine against C region gene of SARS-CoV S protein, and observe the immune response induced by DNA vaccine injection and mucosal immunization. Methods: A recombinant DNA vaccine plasmid was constructed by cloning Sc gene fragment into pcDNA3. 1( - ) ,which could induce anti-SARS-CoV specific antibodies in mouse. After mice immunized with the recombinant DNA vaccine by injection and mucosal immunization, the SARS-CoV antibodies in the sera of mice were detected by ELISA,the lymphocyte phenotypes were detected by FCM,the specific antigen expressed in tissues was detected with immuno histochemical analysis. Detect spleen and MLN lymphocyte proliferation and CD8+ CTL response in vitro. Results: The titer of anti-Sc IgG was much higher in the mouse injected pcDNA3. 1-Sc than that of control group (P
