The effects of protein kinase C alpha cDNA on the expression of genes of multidrug resistance in renal cell carcinoma 786-0 cell line
- VernacularTitle:蛋白激酶C?-cDNA对肾癌细胞786-0多药耐药基因表达的影响
- Author:
Lizhuo LI
;
Chuize KONG
;
Tao LIU
;
Gefei LIU
- Publication Type:Journal Article
- Keywords:
Carcinoma,renal cell;
Protein kinase C;
Multidrug resistance
- From:
Chinese Journal of Urology
2000;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of protein kinase C alpha(PKC?) cDNA on the expression of genes of multidrug resistance (MDR) in renal cell carcinoma(RCC) 786-0 cell line.Methods LR/BR recombination reactions were used to generate mammalian expression vectors of PKC? cDNA with C-terminal fused green fluorescent protein(GFP),and vectors were transfected into human RCC cell with Lipofectimine 2000.Western blot method and inverted fluorescent microscope were used to determine the expression of PKC? in RCC cells transfected by PKC? cDNA.RT-PCR was used to determine the expression of MDR-related genes MDR1,MRP1 and LRP in RCC cells transfected by PKC? cDNA.Results After transfection of 786-0 cells with pcDNA-DEST47-PKC?-GFP vectors,the results of Western blot showed that PKC? was highly expressed in human RCC 786-0 cells;and inverted fluorescent microscopy showed that GFP was highly expressed in RCC 786-0 cells.The results of semi-quantitative RT-PCR analysis showed that the expression level of MDR1 was higher in RCC cells transfected by PKC? cDNA than in RCC cells.Conclusions The expression of MDR1 mRNA in 786-0 cell line can be up-regulated by PKC? cDNA,which suggests PKC? cDNA can induce the increase of MDR in renal cell carcinoma.
