The study of spermatogenesis impairment of contralateral testicle following unilateral testicular torsion and immune response
- VernacularTitle:单侧睾丸扭转引发对侧睾丸生精损伤免疫机制的实验研究
- Author:
Ziming LIU
;
Xinmin ZHENG
;
Zhiwei YANG
;
Shiwen LI
;
Liquan HU
- Publication Type:Journal Article
- Keywords:
Spermatic cord torsion;
Autoimmunity;
Apoptosis;
Flow cytometry
- From:
Chinese Journal of Urology
2000;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of contralateral testicle spermatogenesis impairment following unilateral testicular torsion in rats.Methods Healthy male Sprague-Dawley rats(n=24) were randomly divided into 3 groups:group 1(sham-operation),2 and 3,each comprising 8 rats.Under surgical conditions,the left testes of the rats in groups 2 and 3 were rotated through clockwise 720? torsion,then detorsion after 12 h or 24 h,respectively.The contralateral testes were harvested after 1 month and the relative proportions of germ cells were measured by the flow cytometry(FCM).Anti-rat immunoglobulin G(IgG)antibodies against spermatozoa antigens were detected in contralateral testicular tissue by immunohistochemical method.Results In groups 1,2 and 3,the weight of contralateral testis was(1555.73?(72.34)),(1184.20?101.02) and(783.60?117.93)mg,respectively;the number of apoptotic cells was 53.25?8.61,1622.00?129.31 and 3401.25?179.75,respectively;the positive rates of antisperm antibodies were 0,0.55?0.02 and 0.69?0.03,respectively.There were significant differences in above parameters between groups(P
