PTEN gene mutation in gastric carcinoma
- VernacularTitle:PTEN基因突变与胃癌临床分期相关性的探讨
- Author:
Mingjin ZOU
;
Yang GAO
;
Bing LUO
;
Hua LIANG
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms?Genes,conversion?Genes,PTEN
- From:Chinese Journal of Current Advances in General Surgery
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the mutation frequencies of the exon 5 and the exon 8 of PTEN (phosphatase and tensin homology deleted on chromosome ten) gene in gastric carcinoma and investigate the relationship of the gene mutation and pathological differentiation and clinical stage.Methods:The mutation of exon 5 and exon 8 of PTEN gene was detected in 42 gastric carcinoma samples and the matched adjacent normal gastric mucosa with polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) method.The PCR products of mutant samples were analysed by DNA sequencing technique.Results:The mutation of PTEN was shown in 3 of the 42 gastric carcinoma tissues and in none of the adjacent normal tissues.The mutation rates of PTEN gene in poorly differentiated and well differentiated samples were 12.00% and 0,respectively (P0.05).The mutation rates of PTEN gene in clinical stage Ⅰand Ⅱ (5.88%) had no significant difference with that in clinical stage Ⅲand Ⅳ (8.00%) (P0.05).Conclusion:PTEN gene mutation occurs mainly in poorly differentiated gastric carcinoma tissues,and the mutation rate is not related to pathological differentiation and clinical stage.
