Grape seed polyphenols reverses multidrug resistance of GBC-SD cell lines
- VernacularTitle:葡萄籽多酚体外逆转胆囊癌细胞株GBC-SD多药耐药作用的研究
- Author:
Fenghui YANG
;
Zhanmin WANG
- Publication Type:Journal Article
- Keywords:
Grape seed polyphenols?Multidrug resistance?P-glycoprotein?Gallbladder neoplasms
- From:
Chinese Journal of Current Advances in General Surgery
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of grape seed polyphenols (GSP) reverseing mutidrug resistance of GBC-SD cell lines.Methods:GBC-SD cell lines were used to determine the effect of GSP.MTT assay was adopted to evaluate the cytotoxity,RT-PCR was used to determine MDR1 mRNA,P-gp and cellular adriamycin was measured by flow cytometry.Results:IC50 of chemotherapeutic agents was reduced in GSP treated group (P0.05),GSP inhibited the expression of P-gh and MDR1 mRNA (P0.05) ,increased celluar accumulation of adriamycin (P0.05).Conclusions:GSP can reverse mutidrug resistance of GBC-SD cell lines,the potential mechanisms is that GSP decreases expression of P-gp、bcl-2 and MDR1 mRNA.
