Effects of lipoxygenase inhibitor NDGA on telomerase and bcl-2 in breast cancer MCF-7 cells
- VernacularTitle:脂氧合酶抑制剂NDGA对乳腺癌MCF-7细胞端粒酶和bcl-2的影响
- Author:
Huantao LIU
;
Jingzhong SUN
- Publication Type:Journal Article
- Keywords:
Breast neoplasms?Lipoxygenase inhibitors?Telomerase?Apoptosis?Genes,bcl-2
- From:Chinese Journal of Current Advances in General Surgery
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of lipoxygenase inhibitor NDGA on telomerase and bcl-2 of breast cancer MCF-7 cells.Methods:MCF-7 cells in the logarithmic growth state were selected and measured.Four cell groups (concentration:0,1,10,100?mol/L)were divided in the experiment.After 48h treatment of NDGA,the mRNA expression of telomerase was examined by RT-PCR and the expression of bcl-2 was measured by flow cytometry.Results:After 48h treatment of different concentration of NDGA,the expressions of hTERTmRNA and bcl-2 in NDGA groups were both significantly lower than the control group (P0.001).Conclusion:NDGA can prevent proliferation and induce apoptosis in MCF-7 cells by means of the down-regulated expressions of hTERTmRNA and bcl-2.
