Apoptosis of lung adenocarcinoma SPC-A1 cells induced by Livin isoform-specific gene silencing
- VernacularTitle:Livin异构体特异性基因沉默诱导肺腺癌SPC-A1细胞凋亡
- Author:
Jianguo SUN
;
Rongxia LIAO
;
Zhengtang CHEN
- Publication Type:Journal Article
- Keywords:
inhibitor of apoptosis family of protein;
Livin;
apoptosis;
gene-silencing;
RNA interference;
non-small-cell lung cancer
- From:
China Oncology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To explore different functions and values of Livin ? and Livin ?-specific silencing in inducing SPC-A1 cell apoptosis, respectively. Methods:First, Livin ?+?, Livin?and Livin ?-specific siRNA were steadily expressed in SPC-A1 respectively. Next, after isoform-specific gene silencing, biological changes with regard to cell morphology and proliferation of lung cancer cell were observed. Then, TUNEL and FCAS were performed to test the rate of apoptosis. Lastly, statistical analysis was performed to explore the different functions and values of isoform-specific gene silencing in inducing lung cancer cell apoptosis.Results:After gene silencing of Livin ?+?, Livin ? and Livin ?,there was an evident decrease in colony forming ability of SPC-A1 compared with the control(P
