Inhibitory effects of shRNA targeting CD44v3 on invasion behavior of human colon cancer cell SW480 in vitro induced by hyaluronan
- VernacularTitle:CD44v3短发夹RNA对透明质酸诱导人结肠癌SW480细胞体外粘附和侵袭的抑制作用
- Author:
Wenlei ZHUO
;
Yan WANG
;
Zhengtang CHEN
- Publication Type:Journal Article
- Keywords:
RNA interference;
CD44v3;
adhesion;
invasion;
SW480 cell line
- From:
China Oncology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To investigate the effect of short hairpin RNA (shRNA) targeting CD44v3 on adhesion and invasion behavior of human colon cancer cell line SW480 induced by hyaluronan in vitro. Methods:RNA interference plasmid including U6 promoter and expressing shRNA of CD44v3 was designed, constructed, and transfected into SW480 cell line. CD44v3 expression was examined by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Adhesive and invasive ability were examined by plate adhesion model and Boyden chamber model.Results:After plasmid transfection,CD44v3 expression in SW480 cell and the number of SW480 cell induced by hyaluronan adhesiving on plate or permeating septum of Boyden chamber decreased significantly (P
