The changes of core protein of proteoglycan and cytokines in the vertebral cartilage endplate of adolescent idiopathic scoliosis
- VernacularTitle:青少年特发性脊柱侧凸椎体软骨终板核心蛋白和细胞因子的变化
- Author:
Hongguang XU
;
Guixing QIU
;
Yipeng WANG
- Publication Type:Journal Article
- Keywords:
Scoliosis;
Transforming growth factor beta;
Fibroblast growth factor 2;
Immunohistochemistry
- From:
Chinese Journal of Orthopaedics
1998;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of the transforming growth factor beta 1, the basic fibroblast growth factor and the core protein of proteoglycan on the convex and concave sides of apex and end vertebral cartilagnous endplate in adolescent idiopathic scoliosis. Methods There were 12 cases in the study group. There were 4 males and 8 females. The age of patients at the surgery ranged from 12 to 20 years(with an average of 14.9 years). The Cobb angle ranged 43? to 102? (mean, 65.1?) preoperatively. The diagnosis of idiopathic scolisis was made based on radiography, CTM and/or MRI to rule out congenital, neuromyeputhic and other scolisis. All patients underwent anterior correction procedures. The sections of the convex and concave side of the resected apex and end vertebral cartilage endplate were examined with S-P immunohistochemistry and the results were analyzed with image analysis system. Results The transforming growth factor beta 1, the basic fibroblast growth factor and the core protein of proteoglycan were all expressed in the cytoplasm of chondrocytes in the cartilaginous endplate. With non-parameter Wilcoxon rank test, the area density and quantity density of the transforming growth factor beta 1, the basic fibroblast growth factor on the concave curve of the apex and end vertebrae are expressed in a significantly higher level than those of the convex curve at the apex and end vertebrae(P0.05). Conclusion There was a significantly higher expression of TGF?1 and bFGF, while a lower expression of the core protein on the concave side, which suggest a possible aetiological factor or a secondary change in the development of AIS.
