The studies on the properties of dendritic cells from human cord blood and malignant pleural effusions
- VernacularTitle:脐血与恶性胸腔积液来源树突状细胞的培养及特性的比较研究
- Author:
Hui HUANG
;
Bohang ZENG
;
Jingqi CHEN
- Publication Type:Journal Article
- Keywords:
Dendritic cells(DCs);
Interleukin- 4(IL- 4);
Granulocyte- macrophage colony stimulating factor (GM- CSF);
Tumor necrosis factor- ?(TNF- ?)
- From:
Cancer Research and Clinic
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the culture and proliferation of dendritic cells from human cord blood and malignant pleural effusions and identify the phenotype and the function of them in vitro. Methods Existing DCs were obtained from cord blood and malignant pleural effusions. The DCs were cultured with IL-4, GM-CSF and TNF-?. The morphological properties of DCs were observed by invert optical microscope. The phenotypes of DCs were analysed by flow cytometry. The proliferative bioactivities of T cells activated by DCs were observed with mixed lymphocyte reaction (MLR). Results No remarkable differences were found in the morphology and cellular phenotype of the DC, derived from two different sources, but there was remarkable difference between their culture time. The DCs from two different sources could activate homologous lymphocytes to proliferate mostly. But the capacity to stimulate the proliferation of homologous lymphocytes had remarkable difference. Conclusion Mature DCs could be induced from cord blood and malignant pleural effusions.
