Effects of bone marrow stromal cells derived neural stem cells on the changes of apoptosis and correlative proteins in rats after focal cerebral ischemia and reperfusion
- VernacularTitle:探讨脑缺血大鼠骨髓基质细胞移植后减少神经细胞凋亡的作用
- Author:
Yonghai LIU
;
Yucheng SONG
;
Lianhua ZHAO
- Publication Type:Journal Article
- Keywords:
Bone marrow cells;
Cerebral ischemia;
Cell transplantation
- From:
Chinese Journal of Organ Transplantation
2005;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of bone marrow stromal cells derived neural stem cells on apoptosis and the expression of Bcl-2 and Bax after focal cerebral ischemia and reperfusion.Methods The model of middle cerebral artery occlusion (MCAO) and reperfusion was set up by Longa. Thirty-two Sprague-Dawley rats were divided into 4 groups: sham-operated group (A), ischemia control group (B), bone marrow stromal cells transplanted group (C) and bone marrow stromal cells derived neural stem cells transplanted group (D). The rats were killed on the day 7 and 14 after transplantation. The brain sections were used for terminal deoxynucleotidyl transferase dUTP mickend labeling (TUNEL) staining and Bcl-2, Bax immunohistochemical staining.Results The number of apoptotic cells in groups C and D was decreased as compared with that in group B on the day 7 and 14 after transplantation (P
